PUBLICATIONS


2022


Discovery of the First Selective Nanomolar Inhibitors of Endoplasmic Reticulum Aminopeptidase 2 by Kinetic Target-Guided Synthesis
Camberlein V, Charlotte FT, Sierocki P, Li L, Gealageas R, Bosc D, Guillaume V, Warenghem S, Leroux F, Rosell M, Cheng K, Medve L, Prigent M, Decanter M, Dumont J, Mpakali A, Giastas P, Herledan A, Couturier C, Haupenthal J, Lesire L, Hirsch AK, Deprez B, Stratikos E, Bouvier M, Deprez-Poulain R*
Angew Chem Int Ed Engl. 2022 Jul 29. doi: 10.1002/anie.202203560

Discovery of Selective Nanomolar Inhibitors for Insulin- Regulated Aminopeptidase Based on α-Hydroxy-β-Amino Acid Derivatives of Bestatin
Vourloumis D, Mavridis I, Athanasoulis A, Temponeras I, Koumantou S, Giastas P, Mpakali A, Magrioti V, Leib J, van Endert P, Stratikos E* and Papakyriakou A*
J Med Chem, 2022, Jul 14. doi: 10.1021/acs.jmedchem.2c00904 

Can ERAP1 and ERAP2 Form Functional Heterodimers? A Structural Dynamics Investigation
Papakyriakou A*, Mpakali A and Stratikos E*
Front Immunol, 2022 Apr 20;13:863529. doi: 10.3389/fimmu.2022.863529 

ERAP2 Inhibition Induces Cell-Surface Presentation by MOLT-4 Leukemia Cancer Cells of Many Novel and Potentially Antigenic PeptidesTemponeras I, Stamatakis G, Samiotaki M, Georgiadis D, Pratsinis H, Panayotou G and Stratikos E*
Int J Mol Sci, 2022 Feb 8;23(3):1913. doi: 10.3390/ijms23031913.

Inhibitor-Dependent Usage of the S1′ Specificity Pocket of ER Aminopeptidase 2
Mpakali A*, Georgiadis D, Stratikos E, and Giastas P*
ACS Med. Chem. Lett. 2022 Jan 13;13(2):218-224. doi: 10.1021/acsmedchemlett.1c00582.


2021


Tunable Hydrogels with Improved Viscoelastic Properties from Hybrid Polypeptides
Skoulas D, Mangiapia G, Parisi D, Kasimatis M, Glynos E, Stratikos E, Vlassopoulos D, Frielinghaus H, and Iatrou H
Macromolecules, 2021, November 16, 2021, doi.org/10.1021/acs.macromol.1c01596.

Allotypic variation in antigen processing controls antigenic peptide generation from SARS-CoV-2 S1 spike glycoprotein.
Stamatakis G, Samiotaki M, Temponeras I, Panayotou G, Stratikos E*.
J Biol Chem. 2021 Oct 22;297(5):101329. doi: 10.1016/j.jbc.2021.101329. Online ahead of print.
PMID: 34688668 Free PMC article.

Conformational dynamics linked to domain closure and substrate binding explain the ERAP1 allosteric regulation mechanism.
Maben Z, Arya R, Georgiadis D, Stratikos E, Stern LJ.
Nat Commun. 2021 Sep 6;12(1):5302. doi: 10.1038/s41467-021-25564-w.
PMID: 34489420 Free PMC article.

The ERAP1 active site cannot productively access the N-terminus of antigenic peptide precursors stably bound onto MHC class I.
Mavridis G, Mpakali A, Zoidakis J, Makridakis M, Vlahou A, Kaloumenou E, Ziotopoulou A, Georgiadis D, Papakyriakou A, Stratikos E*.
Sci Rep. 2021 Aug 13;11(1):16475. doi: 10.1038/s41598-021-95786-x.
PMID: 34389743 Free PMC article.

Discovery of Selective Inhibitor Leads by Targeting an Allosteric Site in Insulin-Regulated Aminopeptidase.
Temponeras I, Chiniadis L, Papakyriakou A, Stratikos E*.
Pharmaceuticals (Basel). 2021 Jun 18;14(6):584. doi: 10.3390/ph14060584.
PMID: 34207179 Free PMC article.

Deficiency of the serine peptidase Kallikrein 6 does not affect the levels and the pathological accumulation of a-synuclein in mouse brain.
Samantha Sykioti V, Karampetsou M, Chalatsa I, Polissidis A, Michael IP, Pagaki-Skaliora M, Nagy A, Emmanouilidou E, Sotiropoulou G, Vekrelli S K.
J Neurochem. 2021 Jun;157(6):2024-2038. doi: 10.1111/jnc.15199. Epub 2020 Oct 19.
PMID: 32974895

Editorial: Physiological, Pathological Roles and Pharmacology of Insulin Regulated Aminopeptidase.
Chai SY, Gutiérrez-de-Terán H, Stratikos E*.
Front Mol Biosci. 2021 Apr 23;8:685101. doi: 10.3389/fmolb.2021.685101. eCollection 2021.
PMID: 33968999 Free PMC article. No abstract available.

A Carbodiimide-Mediated P-C Bond-Forming Reaction: Mild Amidoalkylation of P-Nucleophiles by Boc-Aminals.
Kokkala P, Rajeshkumar T, Mpakali A, Stratikos E, Vogiatzis KD, Georgiadis D.
Org Lett. 2021 Mar 5;23(5):1726-1730. doi: 10.1021/acs.orglett.1c00155. Epub 2021 Feb 22.
PMID: 33617265

ERAP2 Increases the Abundance of a Peptide Submotif Highly Selective for the Birdshot Uveitis-Associated HLA-A29.
Venema WJ, Hiddingh S, de Boer JH, Claas FHJ, Mulder A, den Hollander AI, Stratikos E, Sarkizova S, van der Veken LT, Janssen GMC, van Veelen PA, Kuiper JJW.
Front Immunol. 2021 Feb 25;12:634441. doi: 10.3389/fimmu.2021.634441. eCollection 2021.
PMID: 33717175 Free PMC article.

Structure-function analysis of naturally occurring apolipoprotein A-I L144R, A164S and L178P mutants provides insight on their role on HDL levels and cardiovascular risk.
Gkolfinopoulou C, Soukou F, Dafnis I, Kellici TF, Sanoudou D, Mavromoustakos T, Stratikos E, Chroni A.
Cell Mol Life Sci. 2021 Feb;78(4):1523-1544. doi: 10.1007/s00018-020-03583-y. Epub 2020 Jul 14.
PMID: 32666307

The Role of Antigen Processing and Presentation in Cancer and the Efficacy of Immune Checkpoint Inhibitor Immunotherapy.
Mpakali A*, Stratikos E*.
Cancers (Basel). 2021 Jan 4;13(1):134. doi: 10.3390/cancers13010134.
PMID: 33406696 Free PMC article. Review.

Common allotypes of ER aminopeptidase 1 have substrate-dependent and highly variable enzymatic properties.
Hutchinson JP, Temponeras I, Kuiper J, Cortes A, Korczynska J, Kitchen S, Stratikos E*.
J Biol Chem. 2021 Jan-Jun;296:100443. doi: 10.1016/j.jbc.2021.100443. Epub 2021 Feb 20.
PMID: 33617882 Free PMC article.


2020


Generation of SARS-CoV-2 S1 Spike Glycoprotein Putative Antigenic Epitopes in Vitro by Intracellular Aminopeptidases.
Stamatakis G, Samiotaki M, Mpakali A, Panayotou G, Stratikos E*.
J Proteome Res. 2020 Nov 6;19(11):4398-4406. doi: 10.1021/acs.jproteome.0c00457. Epub 2020 Sep 22.
PMID: 32931291 Free PMC article.

Changes in the cellular fatty acid profile drive the proteasomal degradation of α-synuclein and enhance neuronal survival.
Xylaki M, Boumpoureka I, Kokotou MG, Marras T, Papadimitriou G, Kloukina I, Magrioti V, Kokotos G, Vekrellis K, Emmanouilidou E.
FASEB J. 2020 Nov;34(11):15123-15145. doi: 10.1096/fj.202001344R. Epub 2020 Sep 15.
PMID: 32931072

Structural Basis of Inhibition of Insulin-Regulated Aminopeptidase by a Macrocyclic Peptidic Inhibitor.
Mpakali A, Saridakis E, Giastas P, Maben Z, Stern LJ, Larhed M, Hallberg M, Stratikos E*.
ACS Med Chem Lett. 2020 Jun 2;11(7):1429-1434. doi: 10.1021/acsmedchemlett.0c00172. eCollection 2020 Jul 9.
PMID: 32676150 Free PMC article.

A systematic re-examination of processing of MHCI-bound antigenic peptide precursors by endoplasmic reticulum aminopeptidase 1.
Mavridis G, Arya R, Domnick A, Zoidakis J, Makridakis M, Vlahou A, Mpakali A, Lelis A, Georgiadis D, Tampé R, Papakyriakou A, Stern LJ, Stratikos E*.
J Biol Chem. 2020 May 22;295(21):7193-7210. doi: 10.1074/jbc.RA120.012976. Epub 2020 Mar 17.
PMID: 32184355 Free PMC article.

Intrastriatal Administration of Exosome-Associated Pathological Alpha-Synuclein Is Not Sufficient by Itself to Cause Pathology Transmission.
Karampetsou M, Sykioti VS, Leandrou E, Melachroinou K, Lambiris A, Giannelos A, Emmanouilidou E, Vekrellis K.
Front Neurosci. 2020 Apr 21;14:246. doi: 10.3389/fnins.2020.00246. eCollection 2020.
PMID: 32372894 Free PMC article.

Correction to "Macromolecular Architecture and Encapsulation of the Anticancer Drug Everolimus Control the Self-Assembly of Amphiphilic Polypeptide-Containing Hybrids".
Karatzas A, Haataja JS, Skoulas D, Bilalis P, Varlas S, Apostolidi P, Sofianopoulou S, Stratikos E, Houbenov N, Ikkala O, Iatrou H.
Biomacromolecules. 2020 Apr 13;21(4):1644. doi: 10.1021/acs.biomac.0c00236. Epub 2020 Mar 5.
PMID: 32134634 No abstract available.

Peripheral alpha-synuclein levels in patients with genetic and non-genetic forms of Parkinson's disease.
Emmanouilidou E, Papagiannakis N, Kouloulia S, Galaziou A, Antonellou R, Papadimitriou D, Athanasiadou A, Bozi M, Koros C, Maniati M, Vekrellis K, Ioannou PC, Stefanis L.
Parkinsonism Relat Disord. 2020 Apr;73:35-40. doi: 10.1016/j.parkreldis.2020.03.014. Epub 2020 Mar 22.
PMID: 32234683

Elevated Serum α-Synuclein Levels in Huntington's Disease Patients.
Breza M, Emmanouilidou E, Leandrou E, Kartanou C, Bougea A, Panas M, Stefanis L, Karadima G, Vekrellis K, Koutsis G.
Neuroscience. 2020 Apr 1;431:34-39. doi: 10.1016/j.neuroscience.2020.01.037. Epub 2020 Feb 8.
PMID: 32045629

Targeting the Regulatory Site of ER Aminopeptidase 1 Leads to the Discovery of a Natural Product Modulator of Antigen Presentation.
Liddle J, Hutchinson JP, Kitchen S, Rowland P, Neu M, Cecconie T, Holmes DS, Jones E, Korczynska J, Koumantou D, Lea JD, Nickels L, Pemberton M, Phillipou A, Schneck JL, Sheehan H, Tinworth CP, Uings I, Wojno-Picon J, Young RJ, Stratikos E*.
J Med Chem. 2020 Mar 26;63(6):3348-3358. doi: 10.1021/acs.jmedchem.9b02123. Epub 2020 Mar 9.
PMID: 32109056

The Differential Expression of ERAP1/ERAP2 and Immune Cell Activation in Pre-eclampsia.
Seamon K, Kurlak LO, Warthan M, Stratikos E, Strauss JF 3rd, Mistry HD, Lee ED.
Front Immunol. 2020 Mar 10;11:396. doi: 10.3389/fimmu.2020.00396. eCollection 2020.
PMID: 32210971 Free PMC article.

High discriminatory ability of peripheral and CFSF biomarkers in Lewy body diseases.
Bougea A, Stefanis L, Emmanouilidou E, Vekrelis K, Kapaki E.
J Neural Transm (Vienna). 2020 Mar;127(3):311-322. doi: 10.1007/s00702-019-02137-2. Epub 2020 Jan 7.
PMID: 31912280

Discovery of Selective Inhibitors of Endoplasmic Reticulum Aminopeptidase 1.
Maben Z, Arya R, Rane D, An WF, Metkar S, Hickey M, Bender S, Ali A, Nguyen TT, Evnouchidou I, Schilling R, Stratikos E, Golden J, Stern LJ.
J Med Chem. 2020 Jan 9;63(1):103-121. doi: 10.1021/acs.jmedchem.9b00293. Epub 2019 Dec 30.
PMID: 31841350 Free PMC article.


2019


Mechanism for antigenic peptide selection by endoplasmic reticulum aminopeptidase 1.
Giastas P, Mpakali A, Papakyriakou A, Lelis A, Kokkala P, Neu M, Rowland P, Liddle J, Georgiadis D, Stratikos E*.
Proc Natl Acad Sci U S A. 2019 Dec 16;116(52):26709-16. doi: 10.1073/pnas.1912070116. Online ahead of print.
PMID: 31843903 Free PMC article.

Exploration of zinc-binding groups for the design of inhibitors for the oxytocinase subfamily of M1 aminopeptidases.
Tsoukalidou S, Kakou M, Mavridis I, Koumantou D, Calderone V, Fragai M, Stratikos E, Papakyriakou A, Vourloumis D.
Bioorg Med Chem. 2019 Dec 15;27(24):115177. doi: 10.1016/j.bmc.2019.115177. Epub 2019 Nov 1.
PMID: 31711716

Marcromolecular Architecture and Encapsulation of the Anticancer Drug Everolimus Control the Self-Assembly of Amphiphilic Polypeptide-Containing Hybrids.
Karatzas A, Haataja JS, Skoulas D, Bilalis P, Varlas S, Apostolidi P, Sofianopoulou S, Stratikos E, Houbenov N, Ikkala O, Iatrou H.
Biomacromolecules. 2019 Dec 9;20(12):4546-4562. doi: 10.1021/acs.biomac.9b01331. Epub 2019 Nov 18.
PMID: 31697482

Voltage-Gated Calcium Channels and α-Synuclein: Implications in Parkinson's Disease.
Leandrou E, Emmanouilidou E, Vekrellis K.
Front Mol Neurosci. 2019 Oct 9;12:237. doi: 10.3389/fnmol.2019.00237. eCollection 2019.
PMID: 31649506 Free PMC article. Review.

How is alpha-synuclein cleared from the cell?
Stefanis L, Emmanouilidou E, Pantazopoulou M, Kirik D, Vekrellis K, Tofaris GK.
J Neurochem. 2019 Sep;150(5):577-590. doi: 10.1111/jnc.14704. Epub 2019 May 8.
PMID: 31069800 Review.

Molecular pathways for antigenic peptide generation by ER aminopeptidase 1.
Mpakali A, Maben Z, Stern LJ, Stratikos E*.
Mol Immunol. 2019 Sep;113:50-57. doi: 10.1016/j.molimm.2018.03.026. Epub 2018 Apr 17.
PMID: 29678301 Free PMC article. Review.

Editing the immunopeptidome of melanoma cells using a potent inhibitor of endoplasmic reticulum aminopeptidase 1 (ERAP1).
Koumantou D, Barnea E, Martin-Esteban A, Maben Z, Papakyriakou A, Mpakali A, Kokkala P, Pratsinis H, Georgiadis D, Stern LJ, Admon A, Stratikos E*.
Cancer Immunol Immunother. 2019 Aug;68(8):1245-1261. doi: 10.1007/s00262-019-02358-0. Epub 2019 Jun 20.
PMID: 31222486 Free PMC article.

High-Resolution Crystal Structure of Endoplasmic Reticulum Aminopeptidase 1 with Bound Phosphinic Transition-State Analogue Inhibitor.
Giastas P, Neu M, Rowland P, Stratikos E*.
ACS Med Chem Lett. 2019 Feb 13;10(5):708-713. doi: 10.1021/acsmedchemlett.9b00002. eCollection 2019 May 9.
PMID: 31097987 Free PMC article.

Endoplasmic reticulum aminopeptidases as a double-faced tool to increase or decrease efficiency of antigen presentation in health and disease.
Kuśnierczyk P, Stratikos E*.
Hum Immunol. 2019 May;80(5):277-280. doi: 10.1016/j.humimm.2019.03.011. Epub 2019 Mar 27.
PMID: 30928619 No abstract available.

Functional characterization of CHEK2 variants in a Saccharomyces cerevisiae system.
Delimitsou A, Fostira F, Kalfakakou D, Apostolou P, Konstantopoulou I, Kroupis C, Papavassiliou AG, Kleibl Z, Stratikos E, Voutsinas GE, Yannoukakos D.
Hum Mutat. 2019 May;40(5):631-648. doi: 10.1002/humu.23728. Epub 2019 Mar 9.
PMID: 30851065

Plasma alpha-synuclein levels in patients with Parkinson's disease: a systematic review and meta-analysis.
Bougea A, Stefanis L, Paraskevas GP, Emmanouilidou E, Vekrelis K, Kapaki E.
Neurol Sci. 2019 May;40(5):929-938. doi: 10.1007/s10072-019-03738-1. Epub 2019 Feb 4.
PMID: 30715632

Endoplasmic reticulum aminopeptidase 1 polymorphism Ile276Met is associated with atopic dermatitis and affects the generation of an HLA-C associated antigenic epitope in vitro.
Niepiekło-Miniewska W, Mpakali A, Stratikos E, Matusiak Ł, Narbutt J, Lesiak A, Kuna P, Wilczyńska K, Nowak I, Wiśniewski A, Zwolińska K, Ponińska J, Płoski R, Szepietowski JC, Kuśnierczyk P.
J Eur Acad Dermatol Venereol. 2019 May;33(5):906-911. doi: 10.1111/jdv.15449. Epub 2019 Mar 4.
PMID: 30680818

Intracellular antigen processing by ERAP2: Molecular mechanism and roles in health and disease.
de Castro JAL, Stratikos E*.
Hum Immunol. 2019 May;80(5):310-317. doi: 10.1016/j.humimm.2018.11.001. Epub 2018 Nov 7.
PMID: 30414458 Review.

Metabolism of Scoparone in Experimental Animals and Humans.
Juvonen RO, Novák F, Emmanouilidou E, Auriola S, Timonen J, Heikkinen AT, Küblbeck J, Finel M, Raunio H.
Planta Med. 2019 Apr;85(6):453-464. doi: 10.1055/a-0835-2301. Epub 2019 Feb 8.
PMID: 30736072

Central nervous system tuberculosis reactivation following intravenous iron supplementation.
Karakonstantis S, Emmanouilidou E, Petraki K, Lydakis C.
Int J Mycobacteriol. 2019 Jan-Mar;8(1):104-106. doi: 10.4103/ijmy.ijmy_10_19.
PMID: 30860189

Inhibitors of ER Aminopeptidase 1 and 2: From Design to Clinical Application.
Georgiadis D, Mpakali A, Koumantou D, Stratikos E*.
Curr Med Chem. 2019;26(15):2715-2729. doi: 10.2174/0929867325666180214111849.
PMID: 29446724 Review.


2018


Birth Registry of Women With Systematic Lupus Erythematosus: The Greek Experience.
Ntali S, Pantazi L, Mpoki K, Nikolopoulos D, Fanouriakis A, Kallitsakis I, Papagoras C, Dimopoulou D, Kteniadaki E, Emmanouilidou E, Chania V, Bertsias G.
Mediterr J Rheumatol. 2018 Dec 18;29(4):228-231. doi: 10.31138/mjr.29.4.228. eCollection 2018 Dec.
PMID: 32185333 Free PMC article.

Thermodynamic destabilization and aggregation propensity as the mechanism behind the association of apoE3 mutants and lipoprotein glomerulopathy.
Katsarou M, Stratikos E, Chroni A.
J Lipid Res. 2018 Dec;59(12):2339-2348. doi: 10.1194/jlr.M088732. Epub 2018 Oct 11.
PMID: 30309894 Free PMC article.

Contribution of RAD51D germline mutations in breast and ovarian cancer in Greece.
Konstanta I, Fostira F, Apostolou P, Stratikos E, Kalfakakou D, Pampanos A, Kollia P, Papadimitriou C, Konstantopoulou I, Yannoukakos D.
J Hum Genet. 2018 Nov;63(11):1149-1158. doi: 10.1038/s10038-018-0498-8. Epub 2018 Aug 15.
PMID: 30111881 Clinical Trial.

Aminobenzosuberone Scaffold as a Modular Chemical Tool for the Inhibition of Therapeutically Relevant M1 Aminopeptidases.
Salomon E, Schmitt M, Marapaka AK, Stamogiannos A, Revelant G, Schmitt C, Alavi S, Florent I, Addlagatta A, Stratikos E, Tarnus C, Albrecht S.
Molecules. 2018 Oct 11;23(10):2607. doi: 10.3390/molecules23102607.
PMID: 30314342 Free PMC article.

Neuropsychiatric symptoms and α-Synuclein profile of patients with Parkinson's disease dementia, dementia with Lewy bodies and Alzheimer's disease.
Bougea A, Stefanis L, Paraskevas GP, Emmanouilidou E, Efthymiopoulou E, Vekrelis K, Kapaki E.
J Neurol. 2018 Oct;265(10):2295-2301. doi: 10.1007/s00415-018-8992-7. Epub 2018 Aug 6.
PMID: 30083953

Self-Healing pH- and Enzyme Stimuli-Responsive Hydrogels for Targeted Delivery of Gemcitabine To Treat Pancreatic Cancer.
Bilalis P, Skoulas D, Karatzas A, Marakis J, Stamogiannos A, Tsimblouli C, Sereti E, Stratikos E, Dimas K, Vlassopoulos D, Iatrou H.
Biomacromolecules. 2018 Sep 10;19(9):3840-3852. doi: 10.1021/acs.biomac.8b00959. Epub 2018 Aug 27.
PMID: 30095907

Modulation of β-glucocerebrosidase increases α-synuclein secretion and exosome release in mouse models of Parkinson's disease.
Papadopoulos VE, Nikolopoulou G, Antoniadou I, Karachaliou A, Arianoglou G, Emmanouilidou E, Sardi SP, Stefanis L, Vekrellis K.
Hum Mol Genet. 2018 May 15;27(10):1696-1710. doi: 10.1093/hmg/ddy075.
PMID: 29547959

The role of endoplasmic reticulum aminopeptidase 2 in modulating immune detection of choriocarcinoma.
Warthan MD, Washington SL, Franzese SE, Ramus RM, Kim KR, York TP, Stratikos E, Strauss JF 3rd, Lee ED.
Biol Reprod. 2018 Mar 1;98(3):309-322. doi: 10.1093/biolre/ioy001.
PMID: 29324974 Free PMC article.

Autophagy inhibition promotes SNCA/alpha-synuclein release and transfer via extracellular vesicles with a hybrid autophagosome-exosome-like phenotype.
Minakaki G, Menges S, Kittel A, Emmanouilidou E, Schaeffner I, Barkovits K, Bergmann A, Rockenstein E, Adame A, Marxreiter F, Mollenhauer B, Galasko D, Buzás EI, Schlötzer-Schrehardt U, Marcus K, Xiang W, Lie DC, Vekrellis K, Masliah E, Winkler J, Klucken J.
Autophagy. 2018;14(1):98-119. doi: 10.1080/15548627.2017.1395992. Epub 2018 Jan 15.
PMID: 29198173 Free PMC article.


1996-2017


CSF biomarkers β-amyloid, tau proteins and a-synuclein in the differential diagnosis of Parkinson-plus syndromes.
Constantinides VC, Paraskevas GP, Emmanouilidou E, Petropoulou O, Bougea A, Vekrellis K, Evdokimidis I, Stamboulis E, Kapaki E.
J Neurol Sci. 2017 Nov 15;382:91-95. doi: 10.1016/j.jns.2017.09.039. Epub 2017 Sep 28.
PMID: 29111028

The Role of Conformational Dynamics in Antigen Trimming by Intracellular Aminopeptidases.
Papakyriakou A, Stratikos E.
Front Immunol. 2017 Aug 7;8:946. doi: 10.3389/fimmu.2017.00946. eCollection 2017.
PMID: 28824657 Free PMC article. Review.

Ligand-Induced Conformational Change of Insulin-Regulated Aminopeptidase: Insights on Catalytic Mechanism and Active Site Plasticity.
Mpakali A, Saridakis E, Harlos K, Zhao Y, Kokkala P, Georgiadis D, Giastas P, Papakyriakou A, Stratikos E.
J Med Chem. 2017 Apr 13;60(7):2963-2972. doi: 10.1021/acs.jmedchem.6b01890. Epub 2017 Apr 3.
PMID: 28328206

The Pivotal Role of Protein Phosphorylation in the Control of Yeast Central Metabolism.
Vlastaridis P, Papakyriakou A, Chaliotis A, Stratikos E, Oliver SG, Amoutzias GD.
G3 (Bethesda). 2017 Apr 3;7(4):1239-1249. doi: 10.1534/g3.116.037218.
PMID: 28250014 Free PMC article.

Critical Role of Interdomain Interactions in the Conformational Change and Catalytic Mechanism of Endoplasmic Reticulum Aminopeptidase 1.
Stamogiannos A, Maben Z, Papakyriakou A, Mpakali A, Kokkala P, Georgiadis D, Stern LJ, Stratikos E.
Biochemistry. 2017 Mar 14;56(10):1546-1558. doi: 10.1021/acs.biochem.6b01170. Epub 2017 Mar 2.
PMID: 28218509 Free PMC article.

Crystal Structures of ERAP2 Complexed with Inhibitors Reveal Pharmacophore Requirements for Optimizing Inhibitor Potency.
Mpakali A, Giastas P, Deprez-Poulain R, Papakyriakou A, Koumantou D, Gealageas R, Tsoukalidou S, Vourloumis D, Mavridis IM, Stratikos E, Saridakis E.
ACS Med Chem Lett. 2017 Feb 21;8(3):333-337. doi: 10.1021/acsmedchemlett.6b00505. eCollection 2017 Mar 9.
PMID: 28337326 Free PMC article.

Optimization and Structure-Activity Relationships of Phosphinic Pseudotripeptide Inhibitors of Aminopeptidases That Generate Antigenic Peptides.
Kokkala P, Mpakali A, Mauvais FX, Papakyriakou A, Daskalaki I, Petropoulou I, Kavvalou S, Papathanasopoulou M, Agrotis S, Fonsou TM, van Endert P, Stratikos E, Georgiadis D.
J Med Chem. 2016 Oct 13;59(19):9107-9123. doi: 10.1021/acs.jmedchem.6b01031. Epub 2016 Sep 20.
PMID: 27606717

The ability of apolipoprotein E fragments to promote intraneuronal accumulation of amyloid beta peptide 42 is both isoform and size-specific.
Dafnis I, Argyri L, Sagnou M, Tzinia A, Tsilibary EC, Stratikos E, Chroni A.
Sci Rep. 2016 Aug 1;6:30654. doi: 10.1038/srep30654.
PMID: 27476701 Free PMC article.

Screening Identifies Thimerosal as a Selective Inhibitor of Endoplasmic Reticulum Aminopeptidase 1.
Stamogiannos A, Papakyriakou A, Mauvais FX, van Endert P, Stratikos E.
ACS Med Chem Lett. 2016 May 31;7(7):681-5. doi: 10.1021/acsmedchemlett.6b00084. eCollection 2016 Jul 14.
PMID: 27437077 Free PMC article.

Exocytosis and Spreading of Normal and Aberrant α-Synuclein.
Emmanouilidou E, Vekrellis K.
Brain Pathol. 2016 May;26(3):398-403. doi: 10.1111/bpa.12373.
PMID: 26940375 Free PMC article. Review.

GABA transmission via ATP-dependent K+ channels regulates α-synuclein secretion in mouse striatum.
Emmanouilidou E, Minakaki G, Keramioti MV, Xylaki M, Balafas E, Chrysanthou-Piterou M, Kloukina I, Vekrellis K.
Brain. 2016 Mar;139(Pt 3):871-90. doi: 10.1093/brain/awv403. Epub 2016 Feb 8.
PMID: 26912647

Structural Basis for Antigenic Peptide Recognition and Processing by Endoplasmic Reticulum (ER) Aminopeptidase 2.
Mpakali A, Giastas P, Mathioudakis N, Mavridis IM, Saridakis E, Stratikos E.
J Biol Chem. 2015 Oct 23;290(43):26021-32. doi: 10.1074/jbc.M115.685909. Epub 2015 Sep 17.
PMID: 26381406 Free PMC article.

Effects of polymorphic variation on the mechanism of Endoplasmic Reticulum Aminopeptidase 1.
Stamogiannos A, Koumantou D, Papakyriakou A, Stratikos E.
Mol Immunol. 2015 Oct;67(2 Pt B):426-35. doi: 10.1016/j.molimm.2015.07.010. Epub 2015 Jul 26.
PMID: 26224046

Crystal Structure of Insulin-Regulated Aminopeptidase with Bound Substrate Analogue Provides Insight on Antigenic Epitope Precursor Recognition and Processing.
Mpakali A, Saridakis E, Harlos K, Zhao Y, Papakyriakou A, Kokkala P, Georgiadis D, Stratikos E.
J Immunol. 2015 Sep 15;195(6):2842-51. doi: 10.4049/jimmunol.1501103. Epub 2015 Aug 10.
PMID: 26259583

Impaired Antiatherogenic Functions of High-density Lipoprotein in Patients with Ankylosing Spondylitis.
Gkolfinopoulou C, Stratikos E, Theofilatos D, Kardassis D, Voulgari PV, Drosos AA, Chroni A.
J Rheumatol. 2015 Sep;42(9):1652-60. doi: 10.3899/jrheum.141532. Epub 2015 Aug 1.
PMID: 26233507

Decreased levels of alpha-synuclein in cerebrospinal fluid of patients with clinically isolated syndrome and multiple sclerosis.
Antonelou RCh, Emmanouilidou E, Gasparinatos G, Velona T, Voumvourakis KI, Stefanis L.
J Neurochem. 2015 Aug;134(4):748-55. doi: 10.1111/jnc.13163. Epub 2015 Jun 11.
PMID: 25962981

3,4-diaminobenzoic acid derivatives as inhibitors of the oxytocinase subfamily of M1 aminopeptidases with immune-regulating properties.
Papakyriakou A, Zervoudi E, Tsoukalidou S, Mauvais FX, Sfyroera G, Mastellos DC, van Endert P, Theodorakis EA, Vourloumis D, Stratikos E.
J Med Chem. 2015 Feb 12;58(3):1524-43. doi: 10.1021/jm501867s. Epub 2015 Jan 30.
PMID: 25635706

Autoimmune disease-associated variants of extracellular endoplasmic reticulum aminopeptidase 1 induce altered innate immune responses by human immune cells.
Aldhamen YA, Pepelyayeva Y, Rastall DP, Seregin SS, Zervoudi E, Koumantou D, Aylsworth CF, Quiroga D, Godbehere S, Georgiadis D, Stratikos E, Amalfitano A.
J Innate Immun. 2015;7(3):275-89. doi: 10.1159/000368899. Epub 2015 Jan 14.
PMID: 25591727 Free PMC article.

The small GTPase Rab11 co-localizes with α-synuclein in intracellular inclusions and modulates its aggregation, secretion and toxicity.
Chutna O, Gonçalves S, Villar-Piqué A, Guerreiro P, Marijanovic Z, Mendes T, Ramalho J, Emmanouilidou E, Ventura S, Klucken J, Barral DC, Giorgini F, Vekrellis K, Outeiro TF.
Hum Mol Genet. 2014 Dec 20;23(25):6732-45. doi: 10.1093/hmg/ddu391. Epub 2014 Aug 4.
PMID: 25092884

A role for naturally occurring alleles of endoplasmic reticulum aminopeptidases in tumor immunity and cancer pre-disposition.
Stratikos E, Stamogiannos A, Zervoudi E, Fruci D.
Front Oncol. 2014 Dec 19;4:363. doi: 10.3389/fonc.2014.00363. eCollection 2014.
PMID: 25566501 Free PMC article. Review.

Regulating adaptive immune responses using small molecule modulators of aminopeptidases that process antigenic peptides.
Stratikos E.
Curr Opin Chem Biol. 2014 Dec;23:1-7. doi: 10.1016/j.cbpa.2014.08.007. Epub 2014 Aug 29.
PMID: 25173825 Review.

In silico modeling of the effects of alpha-synuclein oligomerization on dopaminergic neuronal homeostasis.
Ouzounoglou E, Kalamatianos D, Emmanouilidou E, Xilouri M, Stefanis L, Vekrellis K, Manolakos ES.
BMC Syst Biol. 2014 May 13;8:54. doi: 10.1186/1752-0509-8-54.
PMID: 24885905 Free PMC article.

Molecular basis for increased risk for late-onset Alzheimer disease due to the naturally occurring L28P mutation in apolipoprotein E4.
Argyri L, Dafnis I, Theodossiou TA, Gantz D, Stratikos E, Chroni A.
J Biol Chem. 2014 May 2;289(18):12931-45. doi: 10.1074/jbc.M113.538124. Epub 2014 Mar 18.
PMID: 24644280 Free PMC article.

A possible structural basis behind the pathogenic role of apolipoprotein E hereditary mutations associated with lipoprotein glomerulopathy.
Stratikos E, Chroni A.
Clin Exp Nephrol. 2014 Apr;18(2):225-9. doi: 10.1007/s10157-013-0886-5. Epub 2013 Oct 23.
PMID: 24149834 Review.

Modulating antigen processing for cancer immunotherapy.
Stratikos E.
Oncoimmunology. 2014 Jan 1;3(1):e27568. doi: 10.4161/onci.27568. Epub 2014 Jan 3.
PMID: 24744979 Free PMC article.

Rationally designed inhibitor targeting antigen-trimming aminopeptidases enhances antigen presentation and cytotoxic T-cell responses.
Zervoudi E, Saridakis E, Birtley JR, Seregin SS, Reeves E, Kokkala P, Aldhamen YA, Amalfitano A, Mavridis IM, James E, Georgiadis D, Stratikos E.
Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):19890-5. doi: 10.1073/pnas.1309781110. Epub 2013 Nov 18.
PMID: 24248368 Free PMC article.

Significance of the hydrophobic residues 225-230 of apoA-I for the biogenesis of HDL.
Fotakis P, Tiniakou I, Kateifides AK, Gkolfinopoulou C, Chroni A, Stratikos E, Zannis VI, Kardassis D.
J Lipid Res. 2013 Dec;54(12):3293-302. doi: 10.1194/jlr.M043489. Epub 2013 Oct 12.
PMID: 24123812 Free PMC article.

Endoplasmic reticulum aminopeptidase-1 alleles associated with increased risk of ankylosing spondylitis reduce HLA-B27 mediated presentation of multiple antigens.
Seregin SS, Rastall DP, Evnouchidou I, Aylsworth CF, Quiroga D, Kamal RP, Godbehere-Roosa S, Blum CF, York IA, Stratikos E, Amalfitano A.
Autoimmunity. 2013 Dec;46(8):497-508. doi: 10.3109/08916934.2013.819855. Epub 2013 Sep 13.
PMID: 24028501 Free PMC article.

Role of the hydrophobic and charged residues in the 218-226 region of apoA-I in the biogenesis of HDL.
Fotakis P, Kateifides AK, Gkolfinopoulou C, Georgiadou D, Beck M, Gründler K, Chroni A, Stratikos E, Kardassis D, Zannis VI.
J Lipid Res. 2013 Dec;54(12):3281-92. doi: 10.1194/jlr.M038356. Epub 2013 Aug 29.
PMID: 23990662 Free PMC article.

Deregulation of calcium homeostasis mediates secreted α-synuclein-induced neurotoxicity.
Melachroinou K, Xilouri M, Emmanouilidou E, Masgrau R, Papazafiri P, Stefanis L, Vekrellis K.
Neurobiol Aging. 2013 Dec;34(12):2853-65. doi: 10.1016/j.neurobiolaging.2013.06.006. Epub 2013 Jul 25.
PMID: 23891486

The diagnostic value of CSF α-synuclein in the differential diagnosis of dementia with Lewy bodies vs. normal subjects and patients with Alzheimer's disease.
Kapaki E, Paraskevas GP, Emmanouilidou E, Vekrellis K.
PLoS One. 2013 Nov 25;8(11):e81654. doi: 10.1371/journal.pone.0081654. eCollection 2013.
PMID: 24282614 Free PMC article.

Antigenic peptide trimming by ER aminopeptidases--insights from structural studies.
Stratikos E, Stern LJ.
Mol Immunol. 2013 Oct;55(3-4):212-9. doi: 10.1016/j.molimm.2013.03.002. Epub 2013 Mar 29.
PMID: 23545452 Free PMC article. Review.

Novel selective inhibitors of aminopeptidases that generate antigenic peptides.
Papakyriakou A, Zervoudi E, Theodorakis EA, Saveanu L, Stratikos E, Vourloumis D.
Bioorg Med Chem Lett. 2013 Sep 1;23(17):4832-6. doi: 10.1016/j.bmcl.2013.07.024. Epub 2013 Jul 23.
PMID: 23916253

A Novel ERAP2 Haplotype Structure in a Chilean Population: Implications for ERAP2 Protein Expression and Preeclampsia Risk.
Vanhille DL, Hill LD, Hilliard DD, Lee ED, Teves ME, Srinivas S, Kusanovic JP, Gomez R, Stratikos E, Elovitz MA, Romero R, Strauss JF 3rd.
Mol Genet Genomic Med. 2013 Jul 1;1(2):98-107. doi: 10.1002/mgg3.13.
PMID: 24040622 Free PMC article.

Allele-dependent thermodynamic and structural perturbations in ApoE variants associated with the correction of dyslipidemia and formation of spherical ApoE-containing HDL particles.
Georgiadou D, Chroni A, Drosatos K, Kypreos KE, Zannis VI, Stratikos E.
Atherosclerosis. 2013 Feb;226(2):385-91. doi: 10.1016/j.atherosclerosis.2012.11.013. Epub 2012 Nov 23.
PMID: 23228878 Free PMC article.

ApoA-IV promotes the biogenesis of apoA-IV-containing HDL particles with the participation of ABCA1 and LCAT.
Duka A, Fotakis P, Georgiadou D, Kateifides A, Tzavlaki K, von Eckardstein L, Stratikos E, Kardassis D, Zannis VI.
J Lipid Res. 2013 Jan;54(1):107-15. doi: 10.1194/jlr.M030114. Epub 2012 Nov 6.
PMID: 23132909 Free PMC article.

Thermodynamic and structural destabilization of apoE3 by hereditary mutations associated with the development of lipoprotein glomerulopathy.
Georgiadou D, Stamatakis K, Efthimiadou EK, Kordas G, Gantz D, Chroni A, Stratikos E.
J Lipid Res. 2013 Jan;54(1):164-76. doi: 10.1194/jlr.M030965. Epub 2012 Oct 30.
PMID: 23110818 Free PMC article.

A common single nucleotide polymorphism in endoplasmic reticulum aminopeptidase 2 induces a specificity switch that leads to altered antigen processing.
Evnouchidou I, Birtley J, Seregin S, Papakyriakou A, Zervoudi E, Samiotaki M, Panayotou G, Giastas P, Petrakis O, Georgiadis D, Amalfitano A, Saridakis E, Mavridis IM, Stratikos E.
J Immunol. 2012 Sep 1;189(5):2383-92. doi: 10.4049/jimmunol.1200918. Epub 2012 Jul 25.
PMID: 22837489 Free PMC article.

The crystal structure of human endoplasmic reticulum aminopeptidase 2 reveals the atomic basis for distinct roles in antigen processing.
Birtley JR, Saridakis E, Stratikos E, Mavridis IM.
Biochemistry. 2012 Jan 10;51(1):286-95. doi: 10.1021/bi201230p. Epub 2011 Dec 9.
PMID: 22106953

Pathological roles of α-synuclein in neurological disorders.
Vekrellis K, Xilouri M, Emmanouilidou E, Rideout HJ, Stefanis L.
Lancet Neurol. 2011 Nov;10(11):1015-25. doi: 10.1016/S1474-4422(11)70213-7.
PMID: 22014436 Review.

A simple approach for human recombinant apolipoprotein E4 expression and purification.
Argyri L, Skamnaki V, Stratikos E, Chroni A.
Protein Expr Purif. 2011 Oct;79(2):251-7. doi: 10.1016/j.pep.2011.06.011. Epub 2011 Jun 25.
PMID: 21712092

Human cytomegalovirus microRNA miR-US4-1 inhibits CD8(+) T cell responses by targeting the aminopeptidase ERAP1.
Kim S, Lee S, Shin J, Kim Y, Evnouchidou I, Kim D, Kim YK, Kim YE, Ahn JH, Riddell SR, Stratikos E, Kim VN, Ahn K.
Nat Immunol. 2011 Sep 4;12(10):984-91. doi: 10.1038/ni.2097.
PMID: 21892175 Free PMC article.

Structural basis for antigenic peptide precursor processing by the endoplasmic reticulum aminopeptidase ERAP1.
Nguyen TT, Chang SC, Evnouchidou I, York IA, Zikos C, Rock KL, Goldberg AL, Stratikos E, Stern LJ.
Nat Struct Mol Biol. 2011 May;18(5):604-13. doi: 10.1038/nsmb.2021. Epub 2011 Apr 10.
PMID: 21478864 Free PMC article.

Probing the S1 specificity pocket of the aminopeptidases that generate antigenic peptides.
Zervoudi E, Papakyriakou A, Georgiadou D, Evnouchidou I, Gajda A, Poreba M, Salvesen GS, Drag M, Hattori A, Swevers L, Vourloumis D, Stratikos E.
Biochem J. 2011 Apr 15;435(2):411-20. doi: 10.1042/BJ20102049.
PMID: 21314638 Free PMC article.

Cutting Edge: Coding single nucleotide polymorphisms of endoplasmic reticulum aminopeptidase 1 can affect antigenic peptide generation in vitro by influencing basic enzymatic properties of the enzyme.
Evnouchidou I, Kamal RP, Seregin SS, Goto Y, Tsujimoto M, Hattori A, Voulgari PV, Drosos AA, Amalfitano A, York IA, Stratikos E.
J Immunol. 2011 Feb 15;186(4):1909-13. doi: 10.4049/jimmunol.1003337. Epub 2011 Jan 17.
PMID: 21242517 Free PMC article.

Biophysical analysis of apolipoprotein E3 variants linked with development of type III hyperlipoproteinemia.
Georgiadou D, Chroni A, Vezeridis A, Zannis VI, Stratikos E.
PLoS One. 2011;6(11):e27037. doi: 10.1371/journal.pone.0027037. Epub 2011 Nov 1.
PMID: 22069485 Free PMC article.

Assessment of α-synuclein secretion in mouse and human brain parenchyma.
Emmanouilidou E, Elenis D, Papasilekas T, Stranjalis G, Gerozissis K, Ioannou PC, Vekrellis K.
PLoS One. 2011;6(7):e22225. doi: 10.1371/journal.pone.0022225. Epub 2011 Jul 14.
PMID: 21779395 Free PMC article.

An apolipoprotein E4 fragment can promote intracellular accumulation of amyloid peptide beta 42.
Dafnis I, Stratikos E, Tzinia A, Tsilibary EC, Zannis VI, Chroni A.
J Neurochem. 2010 Nov;115(4):873-84. doi: 10.1111/j.1471-4159.2010.06756.x.
PMID: 20412390 Free PMC article.

Placental leucine aminopeptidase efficiently generates mature antigenic peptides in vitro but in patterns distinct from endoplasmic reticulum aminopeptidase 1.
Georgiadou D, Hearn A, Evnouchidou I, Chroni A, Leondiadis L, York IA, Rock KL, Stratikos E.
J Immunol. 2010 Aug 1;185(3):1584-92. doi: 10.4049/jimmunol.0902502. Epub 2010 Jun 30.
PMID: 20592285 Free PMC article.

Cell-produced alpha-synuclein oligomers are targeted to, and impair, the 26S proteasome.
Emmanouilidou E, Stefanis L, Vekrellis K.
Neurobiol Aging. 2010 Jun;31(6):953-68. doi: 10.1016/j.neurobiolaging.2008.07.008. Epub 2008 Aug 20.
PMID: 18715677

Cell-produced alpha-synuclein is secreted in a calcium-dependent manner by exosomes and impacts neuronal survival.
Emmanouilidou E, Melachroinou K, Roumeliotis T, Garbis SD, Ntzouni M, Margaritis LH, Stefanis L, Vekrellis K.
J Neurosci. 2010 May 19;30(20):6838-51. doi: 10.1523/JNEUROSCI.5699-09.2010.
PMID: 20484626 Free PMC article.

A continuous fluorigenic assay for the measurement of the activity of endoplasmic reticulum aminopeptidase 1: competition kinetics as a tool for enzyme specificity investigation.
Evnouchidou I, Berardi MJ, Stratikos E.
Anal Biochem. 2009 Dec 1;395(1):33-40. doi: 10.1016/j.ab.2009.07.032. Epub 2009 Jul 26.
PMID: 19638272

Inducible over-expression of wild type alpha-synuclein in human neuronal cells leads to caspase-dependent non-apoptotic death.
Vekrellis K, Xilouri M, Emmanouilidou E, Stefanis L.
J Neurochem. 2009 Jun;109(5):1348-62. doi: 10.1111/j.1471-4159.2009.06054.x. Epub 2009 Mar 23.
PMID: 19476547

A new role for Zn(II) aminopeptidases: antigenic peptide generation and destruction.
Evnouchidou I, Papakyriakou A, Stratikos E.
Curr Pharm Des. 2009;15(31):3656-70. doi: 10.2174/138161209789271816.
PMID: 19925418 Review.

Biophysical analysis of progressive C-terminal truncations of human apolipoprotein E4: insights into secondary structure and unfolding properties.
Chroni A, Pyrpassopoulos S, Thanassoulas A, Nounesis G, Zannis VI, Stratikos E.
Biochemistry. 2008 Sep 2;47(35):9071-80. doi: 10.1021/bi800469r. Epub 2008 Aug 9.
PMID: 18690708 Free PMC article.

The internal sequence of the peptide-substrate determines its N-terminus trimming by ERAP1.
Evnouchidou I, Momburg F, Papakyriakou A, Chroni A, Leondiadis L, Chang SC, Goldberg AL, Stratikos E.
PLoS One. 2008;3(11):e3658. doi: 10.1371/journal.pone.0003658. Epub 2008 Nov 6.
PMID: 18987748 Free PMC article.

Rapid genotyping of CYP2D6, CYP2C19 and TPMT polymorphisms by primer extension reaction in a dipstick format.
Litos IK, Emmanouilidou E, Glynou KM, Laios E, Ioannou PC, Christopoulos TK, Kampa M, Castanas E, Gravanis A.
Anal Bioanal Chem. 2007 Nov;389(6):1849-57. doi: 10.1007/s00216-007-1593-4. Epub 2007 Oct 2.
PMID: 17909762

High-throughput chemiluminometric determination of prostate-specific membrane antigen mRNA in peripheral blood by RT-PCR using a synthetic RNA internal standard.
Emmanouilidou E, Ioannou PC, Christopoulos TK.
Anal Bioanal Chem. 2004 Sep;380(1):90-7. doi: 10.1007/s00216-004-2719-6. Epub 2004 Jul 20.
PMID: 15278342

Enhanced catalytic action of HLA-DM on the exchange of peptides lacking backbone hydrogen bonds between their N-terminal region and the MHC class II alpha-chain.
Stratikos E, Wiley DC, Stern LJ.
J Immunol. 2004 Jan 15;172(2):1109-17. doi: 10.4049/jimmunol.172.2.1109.
PMID: 14707085

Determination of prostate specific antigen mRNA in peripheral blood by reverse transcriptase polymerase chain reaction and a simple chemiluminometric hybridization assay in a high-throughput format.
Emmanouilidou E, Ioannou PC, Christopoulos TK, Polizois K.
Anal Biochem. 2003 Feb 1;313(1):97-105. doi: 10.1016/s0003-2697(02)00566-3.
PMID: 12576064

Identification of the lateral interaction surfaces of human histocompatibility leukocyte antigen (HLA)-DM with HLA-DR1 by formation of tethered complexes that present enhanced HLA-DM catalysis.
Stratikos E, Mosyak L, Zaller DM, Wiley DC.
J Exp Med. 2002 Jul 15;196(2):173-83. doi: 10.1084/jem.20020117.
PMID: 12119342 Free PMC article.

Structural similarity of the covalent complexes formed between the serpin plasminogen activator inhibitor-1 and the arginine-specific proteinases trypsin, LMW u-PA, HMW u-PA, and t-PA: use of site-specific fluorescent probes of local environment.
Backovic M, Stratikos E, Lawrence DA, Gettins PG.
Protein Sci. 2002 May;11(5):1182-91. doi: 10.1110/ps.4320102.
PMID: 11967374 Free PMC article.

Imaging Ca2+ concentration changes at the secretory vesicle surface with a recombinant targeted cameleon.
Emmanouilidou E, Teschemacher AG, Pouli AE, Nicholls LI, Seward EP, Rutter GA.
Curr Biol. 1999 Aug 26;9(16):915-8. doi: 10.1016/s0960-9822(99)80398-4.
PMID: 10469598

Formation of the covalent serpin-proteinase complex involves translocation of the proteinase by more than 70 A and full insertion of the reactive center loop into beta-sheet A.
Stratikos E, Gettins PG.
Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):4808-13. doi: 10.1073/pnas.96.9.4808.
PMID: 10220375 Free PMC article.

Change in environment of the P1 side chain upon progression from the Michaelis complex to the covalent serpin-proteinase complex.
Futamura A, Stratikos E, Olson ST, Gettins PG.
Biochemistry. 1998 Sep 22;37(38):13110-9. doi: 10.1021/bi981234m.
PMID: 9748317

Secretory-granule dynamics visualized in vivo with a phogrin-green fluorescent protein chimaera.
Pouli AE, Emmanouilidou E, Zhao C, Wasmeier C, Hutton JC, Rutter GA.
Biochem J. 1998 Jul 1;333 ( Pt 1)(Pt 1):193-9. doi: 10.1042/bj3330193.
PMID: 9639579 Free PMC article.

Mapping the serpin-proteinase complex using single cysteine variants of alpha1-proteinase inhibitor Pittsburgh.
Stratikos E, Gettins PG.
J Biol Chem. 1998 Jun 19;273(25):15582-9. doi: 10.1074/jbc.273.25.15582.
PMID: 9624149

Major proteinase movement upon stable serpin-proteinase complex formation.
Stratikos E, Gettins PG.
Proc Natl Acad Sci U S A. 1997 Jan 21;94(2):453-8. doi: 10.1073/pnas.94.2.453.
PMID: 9012804 Free PMC article.

Recombinant human pigment epithelium-derived factor (PEDF): characterization of PEDF overexpressed and secreted by eukaryotic cells.
Stratikos E, Alberdi E, Gettins PG, Becerra SP.
Protein Sci. 1996 Dec;5(12):2575-82. doi: 10.1002/pro.5560051220.
PMID: 8976566 Free PMC article.