Skip to main navigation
Skip to main content
Skip to page footer
TEACHING
Classes offered
- Contents: 1) Introduction to Biochemistry; Structure of biomolecules: amino acids, proteins, carbohydrates, lipids and nucleic acids. 2) Structure and function of proteins. 3) Organization of biomolecules in high-order structures; Composition, organization and function of cellular membranes; Transport through membranes; DNA and chromatin. 4) Enzyme kinetics and mechanisms of function; Modulation and inhibition of enzymes; Use of enzyme inhibitor and modulators to address problems in medicine and other sciences. 6) Basic principles of metabolism; Glycolysis, gluconeogenesis, citric acid cycles, Oxidative Phosphorylation, Photosynthesis.
- Theory: Storage and flow of the genetic information. Replication, transcription and translation of DNA. Gene regulation and post-translational modifications. Cellular compartmentalization. Special cellular pathways. Protein degradation. Signaling pathways. Methods for analyzing proteins and DNA.
- Laboratory: (A) Analysis of proteins: Fractionation from cellular extracts; Spectroscopic quantifiation; Polyacrylamide gel electrophoresis. (B) Molecular Biology: Bacterial cultures and growth kinetics; DNA isolation from E.coli; DNA quantification and purity determination; Digestion of DNA by restriction endonucleases; DNA analysis on agarose gels
Advanced Topics in Biochemistry (starting Spring semester 2024)
- Contents: Mechanisms of enzymatic regulation. Biochemistry of the immune system. Transport sorting and secretion of proteins. Advanced metabolic processes. Molecular Machines. Drug development.